25B-NBOH (2-(4-Bromo-2,5-dimethoxyphenyl)-N-(2-hydroxybenzyl)ethanamine)
Forensic and Analytical Overview
1. Chemical Identification
Common name: 25B-NBOH
Systematic name: 2-(4-Bromo-2,5-dimethoxyphenyl)-N-(2-hydroxybenzyl)ethanamine
Chemical class: Phenethylamine / NBOH Series
Molecular formula: C₁₇H₂₀BrNO₃
Molecular weight: ~366.25 g/mol
Structural relation: Hydroxybenzyl analogue of 25B-NBOMe, derived from the parent compound 2C-B.
2. Background
25B-NBOH is a synthetic phenethylamine belonging to the NBOH family of compounds, first identified in analytical and toxicological studies during the early 2010s.
Structurally related to 2C-B and 25B-NBOMe, it is of particular interest in research examining the binding behavior of phenethylamine analogues and their differentiation in forensic analysis.
While occasionally detected in seized materials, its primary relevance is in laboratory and reference standard contexts.
3. Analytical Characterization
Established laboratory techniques for identification include:
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Gas Chromatography–Mass Spectrometry (GC–MS)
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Liquid Chromatography–Mass Spectrometry (LC–MS)
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Fourier Transform Infrared Spectroscopy (FTIR)
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Nuclear Magnetic Resonance (NMR) spectroscopy
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High-resolution mass spectrometry (HRMS)
Distinctive bromine isotope peaks and mass fragmentation patterns enable clear confirmation and differentiation from closely related NBOMe analogues.
4. Research and Forensic Relevance
25B-NBOH is primarily utilized in:
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Analytical method development and validation
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Structural comparison within the NBOH/NBOMe series
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Forensic toxicology reference profiling
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Spectroscopic and receptor-binding model studies under controlled laboratory conditions
5. Legal and Safety Notes
Status: Controlled or restricted under analogue legislation in several jurisdictions.
Handling: For use only by authorized research facilities with appropriate licensing.
Storage: Keep sealed, dry, and protected from light at controlled room temperature.
Safety: Laboratory personnel should employ PPE and adhere to standard chemical safety and disposal protocols.
6. Summary
25B-NBOH is a bromine-substituted NBOH analogue used in analytical, forensic, and toxicological research. Its well-defined mass spectral and spectroscopic characteristics support the classification and study of emerging phenethylamine-based substances.
⚠️ For Research Use Only / Not for Human Consumption
Not for medical, veterinary, or household use.
For qualified laboratory professionals only.
